Title of Abstract
Efficient wound healing requires the angiogenic and fibrogenic activity of macrophages, which are derived at least in part from circulating monocytes that undergo differentiation post-extravasation. We focus specifically on unlocking the therapeutic potential of pro-regenerative monocyte subsets and their progeny. Blood monocytes exist in two primary subpopulations, classical and non-classical monocytes, which are distinguished in mice by high and low expression of Ly6C, respectively. While classical Ly6Chi monocytes rapidly extravasate into inflamed tissue and give rise to macrophages, the question of how non-classical Ly6Clo monocytes are also recruited to injured tissues and what functions they subsequently perform fuels great interest. We want to develop of “immunologically smart” biomaterials that can tune the regenerative potential of subpopulations of leukocytes to the needs defined by the injury microenvironment. Our goal is to harness the phenotypic complexity and the division of labor among innate immune cell subsets to amplify endogenous mechanisms of tissue repair. In this presentation, we utilize powerful in situ visualization of monocyte-biomaterial interactions using intravital laser scanning confocal microscopy (LSCM) to assess the regulatory functions of defined monocyte / macrophage populations in the injury niche. We also use labeling techniques of defined blood monocyte subsets to identify and exploit the differentiation bias of non-classical monocytes toward wound-healing macrophages in diverse tissue contexts.
Exciting new advances in drug discovery, computational systems biology and tissue engineering foreshadow a new era in regenerative medicine as breakthrough technologies are made available to address a wide variety of unsolved medical problems. Our lab is focused on the synergistic integration of these important domains in biomedical sciences and engineering for development of effective new strategies to repair, replace, preserve or enhance tissue or organ function.
Cheryl L. San Emeterio; Claire E. Olingy; Yihsuan Chu; Molly E. Ogle; and Edward A. Botchwey
All Author Affiliations
Wallace H. Coulter Department of Biomedical Engineering; Georgia Institute of Technology and Emory University; Atlanta, GA 30332, USA